Development of Cultivation Technology for the Escherichia Coli Recombinant Strain Producing Arginine Deiminase of Mycoplasma Hominis

TitleDevelopment of Cultivation Technology for the Escherichia Coli Recombinant Strain Producing Arginine Deiminase of Mycoplasma Hominis
Publication TypeJournal Article
Year of Publication2014
AuthorsFayura, LR, Boretsky, Yu.R, Pynyaha, Yu.V, Martynyuk, NB, Skorohod, VV, Sybirny, AA
Short TitleSci. innov.
DOI10.15407/scine10.04.029
Volume10
Issue4
SectionResearch and Technical Innovative Projects of the National Academy of Sciences of Ukraine
Pagination29-36
LanguageEnglish
Abstract

The Escherichia coli recombinant strain producing arginine deiminase of Mycoplasma hominis has been designed. Storage conditions that provide stabilization of most productive clones of the producer were found. Terms for cultivation of the arginine deiminase producer using bioreactors of different volume were optimized. Highly purified samples of arginine deiminase were obtained and their long-term storage conditions were selected.

Keywordsarginine deiminase, bacteria Escherichia coli, Mycoplasma hominis, producer
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